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Published Online April 24, 2003
Science DOI: 10.1126/science.1083529

Research Articles

Submitted on February 14, 2003
Accepted on April 16, 2003

Sustained Microtubule Treadmilling in Arabidopsis Cortical Arrays

Sidney L. Shaw 1, Roheena Kamyar 2, David W. Ehrhardt 2*

1 Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
2 Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305, USA.

* To whom correspondence should be addressed. E-mail: ehrhardt{at}andrew2.stanford.edu.

Plant cells create highly structured microtubule arrays at the cell cortex without a central organizing center to anchor the microtubule ends. In vivo imaging of individual microtubules in Arabidopsis plants revealed that new microtubules initiated at the cell cortex and exhibited dynamics at both ends. Polymerization-biased dynamic instability at one end and slow depolymerization at the other resulted in sustained microtubule migration across the cell cortex by a hybrid treadmilling mechanism. This motility caused widespread microtubule repositioning and contributed to changes in array organization through microtubule reorientation and bundling.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)