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Published Online May 9, 2002
Science DOI: 10.1126/science.1071837

Reports

Submitted on March 13, 2002
Accepted on April 25, 2002

Comparative Genome Sequencing for Discovery of Novel Polymorphisms in Bacillus anthracis

Timothy D. Read 1, Steven L. Salzberg 1, Mihai Pop 1, Martin Shumway 1, Lowell Umayam 1, Lingxia Jiang 1, Erik Holtzapple 1, Joseph D. Busch 2, Kimothy L. Smith 2, James M. Schupp 2, Daniel Solomon 2, Paul Keim 2, Claire M. Fraser 1*

1 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
2 Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011, USA.

* To whom correspondence should be addressed. E-mail: cmfraser{at}tigr.org.

Comparison of the whole-genome sequence of Bacillus anthracis isolated from a victim of a recent bioterrorist anthrax attack with a reference reveals 60 new markers that include single nucleotide polymorphisms, indels and tandem repeats. Genome comparison detected four high-quality SNPs between the two sequenced B. anthracis chromosomes and seven differences between different preparations of the reference genome. These markers have been tested on a collection of anthrax isolates, and were found to divide these samples into distinct families. These results demonstrate that genome-based analysis of microbial pathogens will provide a powerful new tool for investigation of infectious disease outbreaks.


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