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Published Online February 7, 2002
Science DOI: 10.1126/science.1068836

Reports

Submitted on December 10, 2001
Accepted on January 25, 2002

Systemic RNAi in C. elegans Requires the Putative Transmembrane Protein SID-1

William M. Winston 1, Christina Molodowitch 1, Craig P. Hunter 1*

1 Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.

* To whom correspondence should be addressed. E-mail: hunter{at}mcb.harvard.edu.

Double-stranded RNA-mediated gene interference (RNAi) in Caenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information is transmitted between cells, we constructed a strain that permits visualization of systemic RNAi. We used this strain to identify systemic RNA interference deficient (sid) loci required to spread gene-silencing information between tissues, but not to initiate or maintain an RNAi response. One of these loci, sid-1, encodes a conserved protein with predicted transmembrane domains. SID-1 is expressed in cells sensitive to RNAi, is localized to the cell periphery, and is required cell-autonomously for RNAi.


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